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data analysis and statistical significance problem

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kdt2006 On August 13, 2007




dundee, United Kingdom
#1New Post! Aug 13, 2007 @ 11:21:56
Hi Everyone,

I am in the process of developing an algorithm for analyzing biological sequences (each length 9). My algorithm extracts all repeated patterns in the list of sequences.

I have a question on the statistical significance of the patterns.

AA..K.V.. (where . represents any character - there are 20 possibilities at each position)

I think the probability for this particular pattern occurring at random is

p = (1*1*20*20*1*20*1*20*20)/(9^20) -Is this right??
8.5e-18

I have 1000 such sequences, is the probability this occurs in the full dataset then simply multiplying the above probability by 1000?

Many thanks
deadlieblackmamba On February 19, 2008




, United Kingdom
#3New Post! Aug 13, 2007 @ 12:06:24
@kdt2006 Said
Hi Everyone,

I am in the process of developing an ...



You lost me about there!
ashu On February 14, 2008




Udupi, India
#4New Post! Jan 23, 2008 @ 12:26:58
The question is not compleately clear to me. What I understood by reading the question is, You have a 9 free places each place can take 20 letters (letters can repeate), And you want to find out the probability of a perticular type of sequence. that is, first and secound letter should be A 5th letter should be K and 7th letter should be V.
You want to find the probability of this perticular sequence.

Your partial answer is correct,
Requred probability is,
p=(number of favorable possibilities)/(total number of possibilities)
i.e. p=(20^5)/(20^9)=(20^-4)

Your calculation of the numerator is correct, similarly you should calculate dinominator too.

And the other part of your question is not clear to me. What you want to find that should be more clear to find the probability. I feel multiplying by 1000 may not be the solution you requred.

Thanks,
Ashu.
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